反应详情

A mixture of 3-{3,5-dimethyl-4-[6-(quinolin-2-ylcarbamoyl)-1H-indol-2-yl]-phenyl}-propionic acid methyl ester (100 mg, 210 μmol) and lithium hydroxide monohydrate (26 mg, 620 μmol) in 5:5:2 THF/MeOH/H2O (10 mL) was stirred at ambient temperature for 18 h. The solution was concentrated to remove the organic solvents, then was diluted with water and neutralized with one equivalent of 1N HCl. The mixture was stirred vigorously for 30 min and the precipitate was filtered and washed with water. The solid was triturated with water and refiltered, then dried at 50° C. under reduced pressure to give the title compound. 1H NMR (DMSO-d6, 400 MHz): δ 12.15 (s, broad, 1H), 11.56 (s, 1H), 10.99 (s, 1H), 8.39 (s, 2H), 8.16 (s, 1H), 7.95 (d, J=7.4 Hz, 1H), 7.88 (d, J=8.3 Hz, 1H), 7.79 (dd, J=8.3, 1.4 Hz, 1H), 7.73 (m, 1H), 7.63 (d, J=8.2 Hz, 1H), 7.52 (t, J=7.1 Hz, 1H), 7.06 (s, 2H), 6.41 (d, J=1.4 Hz, 1H), 2.82 (t, J=7.4 Hz, 2H), 2.57 (t, J=7.6 Hz, 2H), 2.11 (s, 6H). MS (m/z) 464.3 (M+1); Retention time=1.36 min (Method 10).

1. concentrationThe solution was concentrated
2. customto remove the organic solvents
3. stirringThe mixture was stirred vigorously for 30 min
4. filtrationthe precipitate was filtered
5. washwashed with water
6. customThe solid was triturated with water
7. customdried at 50° C. under reduced pressure