Triethylamine → cyclopropanesulfonic acid {3-[(1R,2S,7R,8S)-3-(4-fluoro-benzyl)-6-hydroxy-4-oxo-3-aza-tricyclo [6.2.1.02,7]undec-5-en-5-yl]-1,1-dioxo-1,4-dihydro-1lambda6-thieno[2,3-e][1,2,4]thiadiazin-7-ylmethyl}-amide

Participants

Reaction Conditions

Reaction Steps

To a solution of (1R,2S,7R,8S)-5-(7-aminomethyl-1,1-dioxo-1,4-dihydro-1λ6-thieno[2,3-e][1,2,4]thiadiazin-3-yl)-3-(4-fluoro-benzyl)-6-hydroxy-3-aza-tricyclo[6.2.1.02,7]undec-5-en-4-one (0.10 g, 0.20 mmol) in dichloromethane (3 mL) at 0° C. was added triethylamine (56 μl, 0.40 mmol) and cyclopropanesulfonyl chloride (23 μl, 0.22 mmol). The reaction was stirred at 0° C. for 30 min, then at 25° C. for 2 h. The reaction was quenched with water (10 mL). The resulting mixture was extracted with ethyl acetate (3×10 mL). The organic layers were combined, dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified via flash column chromatography (Teledyne Isco RediSep column; 0-1% methanol in dichloromethane) to afford the desired product, cyclopropanesulfonic acid {3-[(1R,2S,7R,8S)-3-(4-fluoro-benzyl)-6-hydroxy-4-oxo-3-aza-tricyclo[6.2.1.02,7]undec-5-en-5-yl]-1,1-dioxo-1,4-dihydro-1λ6-thieno[2,3-e][1,2,4]thiadiazin-7-ylmethyl}-amide (0.10 g, 0.17 mmol, 84%), as a white solid. 1H NMR (400 MHz, CDCl3) δ: 1.15-1.78 (10H, m), 2.32-2.38 (1H, m), 2.56-2.58 (1H, m), 2.84-2.88 (2H, m), 3.48 (1H, d, J=9.2 Hz), 4.21 (1H, d, J=15.6 Hz), 4.51 (2H, d, J=6.4 Hz), 5.15 (1H, d, J=14.8 Hz), 5.35 (1H, t, J=6.4 Hz), 7.02-7.07 (3H, m), 7.19-7.22 (2H, m). LC-MS (ESI) calculated for C26H27FN4O6S3 606.71. found 607.2 [M+H+].

Provenance & Evidence