反应详情

A solution of crude (85% purity) 2-(4-bromo-2-fluorophenyl)-2-(4-(1-(hydroxymethyl)cyclopropyl)-3-oxo-1-oxa-4,9-diazaspiro[5.5]undecan-9-yl)acetamide (0.488 mmol) in 1,4-dioxane (2 mL) was treated with 7-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)quinoline (0.537 mmol), PdCl2(dppf)-CH2Cl2 adduct (0.024 mmol), and 2M aq potassium carbonate (1.464 mmol). The reaction vessel was purged with nitrogen and sealed, and the mixture irradiated in a Biotage Initiator Microwave at 120° C. for 15 min. Additional portions of 7-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)quinoline (0.117 mmol) and PdCl2(dppf)-CH2Cl2 adduct (0.012 mmol) were added. The reaction vessel was purged with nitrogen and sealed, and the mixture irradiated in a Biotage Initiator Microwave at 120° C. for 5 min. The resulting black mixture was diluted with water (50 mL) and brine (20 mL) and was extracted with ethyl acetate three times. The combined organic layers were treated with Silicycle Si-thiol (20 mg), dried over sodium sulfate, filtered and concentrated in vacuo. The resulting solid was purified by reverse phase HPLC (20-60% acetonitrile/water with 0.1% NH4OH). Fractions containing the desired product were selected, combined and concentrated to dryness in vacuo. Further purification by chiral HPLC (Chromegachiral CC4, methanol) followed by lyophilization from water (w/25% acetonitrile) afforded the title compound as a white solid in >98% ee (43 mg, 16% yield). MS(ES)+ m/e 519.3 [M+H]+. αD=+47 deg (c=0.05, methanol).

1. customThe reaction vessel was purged with nitrogen
2. customsealed
3. customthe mixture irradiated in a Biotage Initiator Microwave at 120° C. for 15 min
4. customThe reaction vessel was purged with nitrogen
5. customsealed
6. customthe mixture irradiated in a Biotage Initiator Microwave at 120° C. for 5 min
7. additionThe resulting black mixture was diluted with water (50 mL) and brine (20 mL)
8. extractionwas extracted with ethyl acetate three times
9. additionThe combined organic layers were treated with Silicycle Si-thiol (20 mg)
10. dry with materialdried over sodium sulfate
11. filtrationfiltered
12. concentrationconcentrated in vacuo
13. customThe resulting solid was purified by reverse phase HPLC (20-60% acetonitrile/water with 0.1% NH4OH)
14. additionFractions containing the desired product
15. concentrationconcentrated to dryness in vacuo
16. customFurther purification by chiral HPLC (Chromegachiral CC4, methanol)