Sulfuric Acid → HCID 71548692, compound

Participants

Reaction Steps

To the product of Step B (40 mg, 0.088 mmol) was added concentrated sulfuric acid (0.5 mL, 9.38 mmol) and the mixture stirred for 16 h. Ice was added followed by saturated aqueous sodium hydrogen carbonate (30 mL) and the mixture was extracted with ethyl acetate (3×10 mL). The combined organic fractions were washed with saturated aqueous brine (1×15 mL), dried with anhydrous sodium sulfate, filtered and the solvent removed in vacuo. The yellow oil was purified by reverse phase HPLC(YMC Pro-C 18 column, gradient elution, 20% to 100% acetonitrile/water with 0.1% TFA) to afford the title compound as a colorless amorphous solid. LC/MS 439.1 (M+1). IC50 value=C rating

Provenance & Evidence