Participants
| Role | Name | HCID | Charge |
|---|
| Reactant | Potassium Chloride | HCID 4873 | — |
| Reactant | 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid | HCID 23831 | — |
| Reactant | D-1,4-Dithiothreitol | HCID 446094 | — |
| Reactant | Magnesium Chloride | HCID 5360315 | — |
| Reactant | 2-Trans,6-trans,10-trans-geranylgeranyl diphosphate(3-) | HCID 5497105 | — |
| Reactant | 2-Trans,6-trans-farnesyl diphosphate(3-) | HCID 15983959 | — |
| Reactant | Geranyl diphosphate(3-) | HCID 19379894 | — |
| Reactant | Neryl diphosphate(3-) | HCID 25245689 | — |
| Reactant | (2Z,6Z)-farnesyl diphosphate | HCID 45479402 | — |
| Solvent | Glycerol | HCID 753 | — |
| Product | (-)-Zingiberene | HCID 92776 | — |
Reaction Steps
Activity assays were performed in 20 mL glass vials in a total volume of 500 μL 50 mM HEPES, pH7.2, 100 mM KCl, 7.5 mM MgCl2, 20 μM MgCl2, 5% (v/v) glycerol, 5 mM DTT with 50 μL protein and either 2 mM cis-FPP (2Z-6Z-farnesyl diphosphate), trans-FPP (E-E-farnesyl diphosphate), GPP (geranyl diphosphate), NDP (neryl diphosphate), or GGPP (geranylgeranyl diphosphate) as a substrate (Echelon Biosciences Incorporated, Salt Lake City, USA). Vials were closed with a teflon lined crimp cap immediately and incubated under moderate shaking for 1 hour at 30° C.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| Patent number | US09090888B2 |
|---|
| Source dataset | uspto-grants-2015_07 |
|---|
| ORD ID | ord-0cf88ee802ff4632b925da4c419ffd8e |
|---|
| Source note | uspto-grants-2015_07 |
|---|
