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To an ice-cooled solution of 2-methyl-2-[4-(2-pyridin-2-yl-4,5-dihydro-6-oxa-1,3a-diazabenzo[e]azulen-9-ylsulfanyl)piperidin-1-yl]propan-1-ol from Example 3 (187 mg, 0.415 mmol) in DCM (20 mL) was added TFA (160 μL, 2.075 mmol) followed by a solution of m-CPBA (79 mg, 0.456 mmol) in DCM (2 mL). The resulting mixture was stirred for 30 min at 0° C. then volatiles were removed under reduced pressure. The crude material was purified by column chromatography (C18, gradient 10-30% MeOH in 0.5% TFA/H2O) and then loaded onto an Isolute® SCX-2 cartridge. The cartridge was washed with MeOH and the product was eluted with 0.5M NH3 in MeOH. Further purification by column chromatography (Si-PCC, gradient 2-17% 2M NH3/MeOH in DCM) afforded 197 as a light brown foam (102 mg, 52%). LCMS: RT1.87 min [M+H]+ 467.1. 1H NMR (DMSO, 400 MHz): δ 8.71 (1H, d, J=2.27 Hz), 8.53 (1H, ddd, J=4.81, 1.82, 0.94 Hz), 8.00 (1H, dt, J=7.92, 1.08 Hz), 7.95 (1H, s), 7.83 (1H, td, J=7.70, 1.85 Hz), 7.50 (1H, dd, J=8.51, 2.30 Hz), 7.26-7.21 (2H, m), 4.58-4.52 (4H, m), 4.18 (1H, t, J=5.42 Hz), 3.21 (2H, d, J=5.19 Hz), 3.07-2.98 (2H, m), 2.72-2.62 (1H, m), 2.17-2.04 (2H, m), 1.78-1.72 (1H, m), 1.48-1.41 (3H, m), 0.89 (6H, s)

1. customwere removed under reduced pressure
2. customThe crude material was purified by column chromatography (C18, gradient 10-30% MeOH in 0.5% TFA/H2O)
3. washThe cartridge was washed with MeOH
4. washthe product was eluted with 0.5M NH3 in MeOH
5. customFurther purification by column chromatography (Si-PCC, gradient 2-17% 2M NH3/MeOH in DCM)