Tromethamine hydrochloride → 2-Amino-2'-deoxyadenosine

Participants

Reaction Steps

The washed cells described above were added to 10 ml of 50 mM Tris-HCl buffer (pH 7.2) containing 100 mM 2′-deoxyribose-1-phosphoric acid and 100 mM 2,6-diaminopurine hemisulfate to a washed cell-concentration of 50 g/ and reacted at 60° C. for 2 hours. The concentration of 2,6-diaminopurine-2′-deoxyriboside produced in the reaction solution was measured by high performance liquid chromatography. The results are shown in Table 1.

Provenance & Evidence