Citric Acid → 3alpha-Hydroxy-21-morpholino-5alpha-pregnane-11,20-dione citrate

Participants

Reaction Steps

3α-Hydroxy-21-morpholino-5α-pregnane-11,20-dione (500 mg) in ethanol (60 ml) was treated with N/10 aqueous citric acid (12 ml) and the mixture was evaporated to dryness. The residue was dried in vacuo to constant weight and the resultant solid dissolved in water (30 ml). The solution was filtered and the solid collected (1 mg) and discarded. Water was added to the filtrate until a concentration of 10 mg/ml with respect to steroid was obtained. The pH of this solution was 3.4. 2N-Aqueous sodium hydroxide was added dropwise until a pH of 4.7 was achieved.

Provenance & Evidence