Hydrochloric Acid → HCID 178501436, compound

Participants

Reaction Steps

21 g of carbobenzoxyglycyl-L-histidine hydrazide is dissolved in 185 ml of 1 N.HCl and to this solution is added 245 ml of ethyl acetate and 4.3 g of NaNO2 at 0° C. with stirring, and after five minutes, 73 ml of a 50% aqueous solution of K2CO3 is added and then this solution is extracted with ethyl acetate. After the extract is dehydrated over anhydrous sodium sulfate, a solution (cooled to 0° C.) of L-leucine methyl ester in ethyl ether (9 g/208 ml) is added to the extract, and this mixture is left to stand overnight at -5° C. The crystals thus produced are filtered off, and washed with ethyl ether to obtain carbobenzoxyglycyl-L-histidyl-L-leucine methyl ester.

Provenance & Evidence