Alprostadil → Pga1

Participants

Reaction Steps

A solution of 170 mg. of trans-Δ2 -PGE1 (prepared as described in Example 2) in 15 ml. of 90% acetic acid was stirred for 17 hours at a temperature of 57° to 60°C. The reaction mixture was concentrated under reduced pressure and the residue dissolved in diethyl ether, washed with water, dried, and concentrated under reduced pressure. The residue was further purified by column chromatography using 20 g. of silica gel and a cyclohexaneethyl acetate mixture (4:1) as eluent. The fraction containing trans-Δ2 -PGA1 was collected and concentrated. The product thus obtained was recrystallised from a cyclohexane-ethyl acetate mixture to obtain pure trans-Δ2 -PGA1 : yield 102 mg. (63%), m.p. 68°C.

Provenance & Evidence