Hydrochloric Acid → HCID 131709942, compound

Participants

Reaction Steps

A solution of 0.975 g of 7β-formylamino-3-(2-thenyl)-ceph-2-em-4ξ-carboxylic acid in 10 ml of dioxane is treated with 1.2 ml of a 1:1-mixture of concentrated hydrochloric acid and water and left to stand for 3 days at room temperature. The faintly yellow reaction solution is lyophilised to yield the 7β-amino-3-(2-thenyl)-ceph-2-em-4ξ-carboxylic acid hydrochloride, which according to thin-layer chromatogram which contains only traces of the starting material; thin-layer chromatogram (silica gel; detection with iodine vapour); Rf = 0.40 (system: n-butanol/acetic acid/water 67:10:23; starting material Rf = 0.56), Rf = 0.51 (system: n-butanol/pyridine/acetic acid/water 40:24:6:30; starting material Rf = 0.60), and Rf = 0.43 (system: ethyl acetate/n-butanol/pyridine/acetic acid/water 42:21:21:6:10; starting material Rf = 0.64).

Provenance & Evidence