Hydrazine hydrate (1:1) → HCID 178453392, compound

Participants

Reaction Steps

3.97 g of Z-Asn-Lys(BOC)-Phe-His-OMe are dissolved in 8 ml of warm dimethylformamide and 12 ml of methanol. 2.5 ml of hydrazine hydrate are added to the solution whilst it is still at about 30°C and the mixture is allowed to stand for 20 hours at room temperature. The peptide-hydrazide is precipitated by adding water, filtered off and washed with water until free of hydrazine. The product is recrystallised from ethanol, melting point = 200°-201°C. In a thin layer chromatogram on silica gel RF43C = 0.5.

Provenance & Evidence