L-Proline 1,1-dimethylethyl ester → N-[(Phenylmethoxy)carbonyl]-L-alanyl-L-proline 1,1-dimethylethyl ester

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Reaction Steps

10.32 g of Z-Ala-ONP and 5.0 g of H-Pro-OtBu in 10 ml of ethyl acetate are allowed to stand for one hour at 0°C and 15 hours at room temperature. The mixture is then diluted with 100 ml of ethyl acetate, washed with 50 % saturated potassium carbonate solution and water, dried over sodium sulphate and evaporated. The resulting oil is homogeneous according to a thin layer chromatogram on silica gel (Rf = 0.65 in the system chloroform-methanol (9:1)) and is directly used further.

Provenance & Evidence