2-keto-L-Gulonic acid → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

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Reaction Steps

This example describes results using a plug flow reactor for preparation of L-ascorbic acid from 2-keto-L-gulonic acid. A solution of 2-KLG was obtained by fermentation of glucose using genetically modified Pantoea citrea followed by partial purification via microfiltration, electrodialysis, and subsequent crystallization which provides the monohydrate of 2-keto-L-gulonic acid. An aqueous solution of 2-KLG was prepared from this material by dissolving 110 g the monohydrate obtained above in 890 mL of water. The resultant solution contained 10.41 wt. % 2-KLG and 0.05 wt. % L-ascorbic acid by analysis. This solution was used as feedstock throughout this experiment.

Provenance & Evidence