beta-Maltose → beta-Maltose

Participants

Reaction Steps

Ten parts by weight of maltose commercialized by Hayashibara Biochemical Laboratories, Inc., Okayama, Japan, was dissolved in 40 parts by weight of water, added at 15° C. and pH7.0 to 2 units/g maltose solid of a purified maltose/trehalose conversion enzyme obtained by the method in Experiment 2, reacted for 48 hours and heated at 100° C. for 10 minutes to inactivate the enzyme. The resultant solution contained about 74% trehalose on dry solid basis. The solution was then decolored with activated carbon, deionized and purified with ion exchanges of H- and OH-forms, concentrated to about 78%, added with 0.1% crystalline trehalose hydrate as seed crystal on dry solid basis and allowed to standing at ambient temperature overnight to effect crystallization. The resultant massecuite was separated and the crystals were sprayed with a minimum amount of water for washing, thus obtaining about 3.0 parts by weight of an extremely pure crystalline trehalose hydrate, purity of 99.8%.

Provenance & Evidence