Participants
| Role | Name | HCID | Charge |
|---|
| Reactant | Thiamine Hydrochloride | HCID 6202 | — |
| Reactant | Calcium Carbonate | HCID 10112 | — |
| Reactant | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| Reactant | Biotin | HCID 171548 | — |
| Reactant | Fumaric Acid | HCID 444972 | — |
| Reactant | Monobasic potassium phosphate | HCID 516951 | — |
| Reactant | Ammonium Sulfate | HCID 6097028 | — |
| Solvent | Acetic Acid | HCID 176 | — |
| Product | L-Tryptophan | HCID 6305 | — |
Reaction Steps
Strains of bacteria of Escherichia coli KB 862-pGH5 disclosed in PCT Application No. (WO 94-08031) were inoculated in a culture medium (pH 6.5) comprising 130 g/L of glucose, 25 g/L of ammonium sulfate, 12 g/L of fumaric acid, 3 ml/L of acetic acid, 1 g/L of KH2PO4, 10 mg/L of MnSO4.7H2O, 1 g/L of MgSO4.7H2O, 10 mg/L of a soybean protein hydrolyzate (as nitrogen), 2 mg/L of thiamine hydrochloride, 50 g/L of calcium carbonate and 0.05 mg/L of biotin, and cultured while agitating at 30° C. for 72 hours to form an L-tryptophan ferment broth. Next, resultant fermentation broth was dried using a laboratory mini-spray dryer to obtain 400 g of a powder having the composition below.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|
| Patent number | US05935635 |
|---|
| Source dataset | uspto-grants-1999_08 |
|---|
| ORD ID | ord-11810352c63747cc9c0c0c97efdb8a18 |
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| Source note | uspto-grants-1999_08 |
|---|
