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A mixture of 10 g of cholesterol, 22 g of oleic acid, 50 ml of isooctane and 200 ml (50,000 U) of an aqueous solution of lipase MY was stirred at 200 rpm for 18 hours. Thereafter, ether was added and the mixture was washed with aqueous sodium bicarbonate to remove the aqueous layer. After several repetitions of this procedure, the ether layer was dried on anhydrous sodium sulfate. The ether was then distilled off to give cholesteryl oleate, which was a white semi-transparent crude product, in 96.3% purity and in a yield of 16.0 g (95.0%).

1. washthe mixture was washed with aqueous sodium bicarbonate
2. customto remove the aqueous layer
3. dry with materialAfter several repetitions of this procedure, the ether layer was dried on anhydrous sodium sulfate
4. distillationThe ether was then distilled off