Glycolic Acid → Formate

Participants

Reaction Conditions

Reaction Steps

Into a 15 mL polypropylene centrifuge tube were placed 3 mL of an aqueous solution containing glycolic acid (3.3 mM), K2HP04 (33 mM, pH 8.0), glycolate oxidase (spinach, 0.33 IU/mL), catalase (bovine liver, 1400 IU/mL), and FMN (0.01 mM). The solution was maintained at 30° C. in air, and 0.15 mL aliquots were removed, filtered through a Millipore 10,000 MW cutoff filter, and analyzed by HPLC. After 1 hour, the yields of glyoxylate, oxalate, and formate were 80.9%, 3.8%, and 0%, respectively, and 15.5% glycolate remained After 2 hours, the yields of glyoxylate, oxalate, and formate were 80.6%, 19.7%, and 0%, respectively, and no glycolate remained.

Provenance & Evidence