Sodium Hydroxide → HCID 70228201, compound

Participants

Reaction Steps

The 4,5-dihydro-1-(3-ethoxy-α-ethylbenzyl)-7-(5-pyrimidinylmethoxy)-[1H]-benz[g]indazole-3-carboxylic acid (600 mg, 1.23 m mol) obtained in Example 34 was suspended in water (20 ml) and dissolved therein by the addition of 1 N sodium hydroxide (2 ml). This solution was subjected to column chromatography using a CHP 20P resin (20 ml; manufactured by Mitsubishi Chemical Co., Ltd.), and eluted successively with water and 50% ethanol-water. After the 50% ethanol-water fraction was concentrated under reduced pressure, the residue was dissolved in water (30 ml) and this solution was concentrated again under reduced pressure. Thereafter, the residue was dissolved in water (15 ml) and freeze-dried to obtain the desired compound (500 mg, 80%) as a colorless powder.

Provenance & Evidence