HCID 53249492, compound → HCID 86752464, compound

HRID 1599966Notes

Reaction equation

HRID 1599966 reaction equation
C1([N:7]2[CH:11]=[NH+:10][N:9](C3C=CC=CC=3)[N:8]2C2C=CC=CC=2)C=CC=CC=1.[Cl-].[C:25]1([N:31]2[N:35]([C:36]3[CH:41]=[CH:40][CH:39]=[CH:38][CH:37]=3)[N:34]=[C:33]([C:42]3[CH:47]=[CH:46][CH:45]=[CH:44][CH:43]=3)[NH2+:32]2)[CH:30]=[CH:29][CH:28]=[CH:27][CH:26]=1>>[NH:8]=[N:7][CH:11]=[N:10][NH2:9].[C:36]1([N:35]2[N:31]([C:25]3[CH:30]=[CH:29][CH:28]=[CH:27][CH:26]=3)[N:32]=[C:33]([C:42]3[CH:43]=[CH:44][CH:45]=[CH:46][CH:47]=3)[NH2+:34]2)[CH:41]=[CH:40][CH:39]=[CH:38][CH:37]=1

Participants

3

Reactant 2

HCID 53249492HCID 53249492, compoundHCID 178420915HCID 178420915, compound

Product 1

HCID 86752464HCID 86752464, compound

Procedure

The present process is conducted on finfish fillets which are believed to be fresh and/or which have been kept on ice. A predetermined weight of a fish fillet, such as 25 gms, is kneaded with a predetermined volume of a liquid growth medium, such as 25 ml, (1:1 ratio) for 2 to 5 minutes at room temperature, e.g., 23° to 25° C., shaking vigorously, and then a predetermined volume of the liquid filtrate, such as 5 ml, is mixed with a small amount, such as 1 ml, of the colorless triphenyl tetrazolium dye salt indicator reagent and allowed to incubate for 10 to 20 minutes at room temperature, e.g., 15°-25° C., shaking vigorously every 5 minutes. The following reaction takes place with the use of 2,3,5-triphenyl tetrazolium chloride as the indicator reagent: ##STR1## This reduction reaction is enabled by the functioning electron transport system of the viable bacterial flora on the fish and proceeds in proportion to the quantity of the enabling bacteria present. The greater the concentration of the red-colored 2,3,5-triphenyl tetrazolium formazan formed in the bacterial cells, the greater the intensity of the color of the formazan solution extracted from the sample.

Workup

  1. waitto incubate for 10 to 20 minutes at room temperature
  2. stirringe.g., 15°-25° C., shaking vigorously every 5 minutes
  3. customThe following reaction
  4. custom##STR1## This reduction reaction

Sources & evidence

ORD record
ord-8c5c4fa3df3f4c489fa1e4878e347e20
Source dataset
uspto-grants-1998_04
Patent number
US05744321

Reaction notes

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