Methanol → HCID 18394993, compound

Participants

Reaction Steps

Sodium hydride (0.1 g) (60% dispersion in mineral oil) was added to dry methanol (50 ml) under an atmosphere of nitrogen at 0° C. The product from step (i) (3.0 g) was added to the above solution and the whole was refluxed for 2 hours. After cooling to room temperature, glacial acetic acid (0.28 ml) was added and the volatiles were then removed under reduced pressure. The residue was dissolved in water (20 ml) and then extracted three times with diethyl ether (20 ml). The aqueous phase was evaporated to dryness under reduced pressure. The resulting solid was triturated with acetone and filtered to leave 5-ethyl-4-thiouridine as a yellow solid (1.39 g).

Provenance & Evidence