Sodium Hydroxide → HCID 135516711, compound

HRID 1604242Notes

Reaction equation

HRID 1604242 reaction equation
[NH2:1][C:2]1[NH:3][C:4](=[O:21])[C:5]2[S:10][C:9](=[O:11])[N:8]([CH2:12][CH2:13][CH2:14][CH2:15][C:16]([O:18]CC)=[O:17])[C:6]=2[N:7]=1.[OH-].[Na+]>CC(O)=O>[NH2:1][C:2]1[NH:3][C:4](=[O:21])[C:5]2[S:10][C:9](=[O:11])[N:8]([CH2:12][CH2:13][CH2:14][CH2:15][C:16]([OH:18])=[O:17])[C:6]=2[N:7]=1

Participants

4

Reactant 2

HCID 14798Sodium HydroxideHCID 135503623HCID 135503623, compound

Product 1

HCID 135516711HCID 135516711, compound

Reagents, catalysts & solvents 1

HCID 176Acetic AcidSolvent

Procedure

A mixture of 5-amino-3-[4-(ethoxycarbonyl)butyl]thiazolo[4,5-d]pyrimidine-2,7(3H,6H)-dione (312 mg, 1 mmol) and 1 N NaOH (5 mL) was stirred at ambient temperature for 1.5 h. The mixture was acidified (pH=4) with AcOH and then refrigerated for 16 h. The solid was collected by filtration, crystallized from a MeOH:H2O mixture and dried under vacuum at 80° C. for 16 h; 220 mg (0.774 mmol, 77%), mp 263-265° C. Ir (KBr): ν3410, 3315 and 3180 (NH, NH2), 1725 (C=O) and 1640 (C=O), and 1640 (C=O, C=N) cm-1. Uv, νmax (nm), (ε×10-3): pH 1, 302 (9.79), 250 (8.52), 222 (31.0); MeOH, 302 (9.48), 250 (8.79), 222 (30.40); pH 11, 292 (8.26), 250 (8.15), 224 (12.20). 1H nmr (DMSO-d6): δ1.43-1.50 (m, 2H, CH2), 1.60-1.67 (m, 2H, CH2), 2.24 (t, 2H, CH2), 3.76 (t, 2H, NCH2), 6.91 (br s, 2H, NH2), 11.1 (br s, 1H, NH), and 12.0 (br s, 1H, COOH) Anal. Calc'd for C10H12N4O4S (284.30): C, 42.25; H, 4.25; N, 19.71. Found: C, 42.32; H, 4.22; N, 19.69.

Workup

  1. waitrefrigerated for 16 h
  2. filtrationThe solid was collected by filtration
  3. customcrystallized from a MeOH
  4. dry with materialH2O mixture and dried under vacuum at 80° C. for 16 h

Sources & evidence

ORD record
ord-c0fa965b1245436abea3aaa8dad16b62
Source dataset
uspto-grants-1999_11
Patent number
US05994321

Reaction notes

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