2-Pyrazinecarboxylic acid → HCID 19603250, compound

Participants

Reaction Steps

To a mixture of 8-[3-[N-[(E)-3-(6-aminopyridin-3-yl)acryloylglycyl]-N-methylamino]-2,6-dichlorobenzyloxy]-2-methylquinoline (90.0 mg), 2-pyrazinecarboxylic acid (24.3 mg) and dimethylformamide (0.9 ml) were added 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (43.9 mg) and 1-hydroxybenzotriazole (35.4 mg). After being stirred for 37 hours at ambient temperature, the mixture was poured into saturated sodium bicarbonate solution and extracted with chloroform. The organic layer was separated, washed with water, dried over magnesium sulfate and evaporated in vacuo. The residue was purified by preparative thin-layer chromatography (chloroform--methanol) to give 8-[2,6-dichloro-3-[N-methyl-N-[(E)-3-[6-(2-pyrazinecarboxamido)-pyridin-3-yl]acryloylglycyl]amino]benzyloxy]-2-methylquinoline (43.7 mg) as a solid.

Provenance & Evidence