Fluorescein 5-isothiocyanate → HCID 131716085, compound

HRID 1606889Notes

Reaction equation

HRID 1606889 reaction equation
O[N:2]1[C:23](=[O:24])[NH:22][C@H:21]2[C@@H:3]1[CH2:4][S:5][C@H:6]2[CH2:7][CH2:8][CH2:9][CH:10](N1C(=O)CCC1=O)[C:11](=[O:13])[OH:12].[CH:25]1[C:30](N=C=S)=[CH:29][C:28]2[C:34]([O:36][C:37]3([C:47]4[CH:48]=[CH:49][C:50]([OH:52])=[CH:51][C:46]=4[O:45][C:39]4[CH:40]=[C:41]([OH:44])[CH:42]=[CH:43][C:38]3=4)[C:27]=2[CH:26]=1)=[O:35]>>[OH:13][C:11]([CH2:10][CH2:9][CH2:8][CH2:7][C@H:6]1[C@@H:21]2[C@@H:3]([NH:2][C:23]([NH:22]2)=[O:24])[CH2:4][S:5]1)=[O:12].[CH:25]1[CH:30]=[CH:29][C:28]([C:34]([OH:36])=[O:35])=[C:27]([C:37]2[C:38]3[CH:43]=[CH:42][C:41]([OH:44])=[CH:40][C:39]=3[O:45][C:46]3[C:47]=2[CH:48]=[CH:49][C:50]([CH:51]=3)=[O:52])[CH:26]=1

Participants

3

Reactant 2

HCID 18730Fluorescein 5-isothiocyanateHCID 129676281N-Hydroxysuccinimido-d-biotin

Product 1

HCID 131716085HCID 131716085, compound

Procedure

The following materials were obtained from Sigma Chemical Co.: avidin, N-hydroxysuccinimidobiotin, fluorescein isothiocyanate, and goat antirabbit IgG IgG, IgG fraction. Rabbit IgG fraction was obtained from Pel-Freez Biologicals. All solvents and buffers were of reagent grade quality. Biotin-fluorescein was synthesized as described above.

Sources & evidence

ORD record
ord-9af045fac68b4128ae9953304e961cbb
Source dataset
uspto-grants-1989_09
Patent number
US04863876

Reaction notes

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