反应详情

Ethyl D-glucopyranoside (30 g, prepared as described in example 10) with an anomeric ratio α:β=1:1 was dissolved in 0.05M acetate buffer (400 ml, pH 4.5) at 30° C. β-glucosidase (50 mg from almonds, Sigma) was added and the mixture was stirred for a week. The solution was evaporated in vacuo and purified by chromatography to give the product as a crystalline solid (8.2 g, 55%).

1. additionwas added
2. customThe solution was evaporated in vacuo
3. custompurified by chromatography