HCID 18650248, compound → HCID 453662, compound

Participants

Reaction Conditions

Reaction Steps

The 4'-α and 4'-β isomers of 3'-deoxy-4'-cyano-5'-O-acetylthymidine (Formula 30) (26 mg, 0.09 mM) is dissolved in 0.2 ml NH4OH/0.2 ml CH3OH and stirred at 24° C. for 2 hours. The solvent is removed by evaporation and the residue chromatographed on silica gel (Preparative TLC, 2 plates, 1000 microns) using 1% CH3OH/EtOAc with 0.8% NH4OH, affording the 4'-α and 4'-β isomers of 3'-deoxy-4'-cyanothymidine, 6 mg, 0.024 mM, MS 251 (M+); and 11 mg, 0.044 mM, MS 251(M+), respectively.

Provenance & Evidence