L-Tyrosine → Levodopa

HRID 1614861Notes

Reaction equation

HRID 1614861 reaction equation
[OH:1][C:2]1[CH:3]=[C:4](N[C@H](C(O)=O)C)[CH:5]=[CH:6][C:7]=1[OH:8].CC(S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O)C.[NH2:30][C@H:31]([C:40]([OH:42])=[O:41])[CH2:32]C1C=CC(O)=CC=1>>[O:41]=[C:40]([C@H:31]([CH2:32][C:4]1[CH:3]=[C:2]([OH:1])[C:7]([OH:8])=[CH:6][CH:5]=1)[NH2:30])[OH:42]

Participants

4

Reactant 3

HCID 6057L-TyrosineHCID 656894isopropyl beta-D-thiogalactopyranoside3×HCID 22818865HCID 22818865, compound3×

Product 1

HCID 6047Levodopa

Procedure

After confirming stationary phase, DOPA (3,4-dihydroxyphenyl-L-alanine) was introduced to the concentration of 1 mM in the medium. After introducing DOPA, inducer IPTG(isopropyl-β-D-thiogalactopyranoside, 1 mM) was added to induce the expression of the above proteins. After adding IPTG, culture was conducted at 37° C. for additional 6 hours. For a positive control, tyrosine was introduced to the concentration of 1 mM instead of DOPA, and the expression of the proteins was induced with IPTG, and for a negative control, nothing was introduced. After culture, the cells were centrifuged at 4000 rpm for 10 minutes, and then, supernatant was removed, and the cells were recovered.

Workup

  1. additionwas introduced to the concentration of 1 mM in the medium
  2. additionwas added
  3. additionfor a negative control, nothing was introduced
  4. waitAfter culture, the cells were centrifuged at 4000 rpm for 10 minutes
  5. customsupernatant was removed
  6. customthe cells were recovered

Sources & evidence

ORD record
ord-f92868cf746c477e95e4d51955ed4812
Source dataset
uspto-grants-2014_07
Patent number
US08765682B2

Reaction notes

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