1-Hydroxybenzotriazole → HCID 49842383, compound

Participants

Reaction Conditions

Reaction Steps

100 mg (0.21 mmol) 2-((3S)-4-(tert-butoxycarbonyl)-3-(cycloheptylmethyl)-6-(3,5-difluorophenyl)-2-oxopiperazin-1-yl)ethanoic acid, 58 mg (0.23 mmol) (R)-5-amino-1,3-dihydrospiro[inden-2,3′-pyrrol[2,3-b]pyridin]-2′(1′H)-one, 56 mg (0.29 mmol) 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide-hydrochloride, 39 mg (0.29 mmol) HOBT, 0.03 ml (0.23 mmol) TEA and 1.50 ml DMF were stirred overnight at RT. The mixture was concentrated by rotary evaporation. The residue was combined with 15 ml of a 1.25M methanolic hydrochloric acid and stirred for 2 h at 50° C. The diastereomers were separated by RP-HPLC. The fractions containing the product were lyophilised.

Provenance & Evidence