Trifluoroacetic acid → (6S)-3-{3-[(8R)-8-(3,5-Difluorophenyl)-6-(2-fluoroethyl)-8-methyl-10-oxo-6,9-diazaspiro[4.5] dec-9-yl]prop-1-yn-1-yl}-5,7-dihydrospiro[cyclopenta[b]pyridine-6,3'-pyrrolo[2,3-b]pyridin]-2'(1'H)-one

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Reaktionsschritte

A solution of (6S)-3-{3-[(8R)-6-(2-chloroethyl)-8-(3,5-difluorophenyl)-8-methyl-10-oxo-6,9-diazaspiro[4.5] dec-9-yl]prop-1-yn-1-yl}-5,7-dihydrospiro[cyclopenta[b]pyridine-6,3′-pyrrolo[2,3-b]pyridin]-2′(1′H)-one from Step A (67 mg, 0.11 mmol) and silver(I)fluoride (140 mg, 1.1 mmol) in acetonitrile (3 ml) was heated at 100° C. for 90 min The mixture was diluted with TFA (0.5 mL), filtered, and purified directly by HPLC using a reversed phase C18 column and eluting with a gradient of H2O:CH3CN:CF3CO2H—90:10:0.1 to 5:95:0.1. The desired fractions were poured onto saturated NaHCO3 (10 mL) and extracted with CH2Cl2 (3×10 ml). The combined organic layers were dried over Na2SO4, filtered and concentrated in vacuo to yield the title compound. MS: m/z=600 (M+1). HRMS: m/z=600.2604 (M+1); calculated m/z=600.2581 (M+1) for C34H34F3N5O2.

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