Acetate → HCID 86638819, compound

HRID 1620373Notes

Reaction equation

HRID 1620373 reaction equation
[C:1]([O-:4])(=[O:3])[CH3:2].[P:5]([O-:9])([OH:8])([OH:7])=[O:6].[Na+:10]>O>[P:5]([O-:9])([OH:8])([OH:7])=[O:6].[Na+:10].[C:1]([O-:4])(=[O:3])[CH3:2].[Na+:10]

Participants

4

Reactant 2

HCID 175Acetate3×HCID 23672064Monosodium phosphate2×

Product 1

HCID 86638819HCID 86638819, compound

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

Batch crystallization was performed by admixing around 10 mL of protein solution with an equal amount of crystallization solution in a 50 mL Falcon tube. 10 mL of the crystallization solution was prepared by admixing acetate buffer, sodium dihydrogen phosphate stock solution and Milli Q water in the tube. In this example, the acetate buffer molarity was 0.1 M, and the acetate buffer pH was around 4.1. Sodium dihydrogen phosphate molarity was 4.2 M. The tube was stored at ambient temperature, agitating the batch on a laboratory shaker. Microscopy of a 1 μL aliquot of the solution was performed multiple times during the following month.

Workup

  1. customBatch crystallization
  2. customwith an equal amount of crystallization solution in a 50 mL Falcon tube
  3. customwas stored at ambient temperature

Sources & evidence

ORD record
ord-2d9b0fefc0aa4477825bf47cc1ec73f8
Source dataset
uspto-grants-2014_07
Patent number
US08772458B2

Reaction notes

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