Acetate → HCID 86638819, compound

HRID 1620374Notes

Reaction equation

HRID 1620374 reaction equation
[C:1]([O-:4])(=[O:3])[CH3:2].[P:5]([O-:9])([OH:8])([OH:7])=[O:6].[Na+:10]>O>[P:5]([O-:9])([OH:8])([OH:7])=[O:6].[Na+:10].[C:1]([O-:4])(=[O:3])[CH3:2].[Na+:10]

Participants

4

Reactant 2

HCID 175Acetate3×HCID 23672064Monosodium phosphate2×

Product 1

HCID 86638819HCID 86638819, compound

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

Batch crystallization was performed by admixing around 50 mL of protein solution with an equal amount of crystallization solution in a clean 1 L polypropylene bottle. 50 mL of the crystallization solution was prepared by admixing acetate buffer, sodium dihydrogen phosphate stock solution and Milli Q water in the tube. In this example, the acetate buffer molarity was 0.1 M, and the acetate buffer pH was around 4.1. Sodium dihydrogen phosphate molarity was 4.2 M. The container was stored at ambient temperature. Microscopy of a 1 μL aliquot of the solution was performed multiple times during the following month.

Workup

  1. customBatch crystallization
  2. customwith an equal amount of crystallization solution in a clean 1 L polypropylene bottle
  3. customwas stored at ambient temperature

Sources & evidence

ORD record
ord-02760d27bff6439ca219ac4378556ec4
Source dataset
uspto-grants-2014_07
Patent number
US08772458B2

Reaction notes

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