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0 °C

Argon was bubbled for 2 min through a glass microwave reaction vessel containing 6-(2-fluoropyridin-3-yl)-2-methyl-9-(tetrahydro-2H-pyran-2-yl)-9H-purine (100 mg, 0.319 mmol) and 5-methoxypyridin-3-amine (47.5 mg, 0.383 mmol, Astatech, Inc, Bristol, Pa.) in THF (5 mL), and the tube was sealed. The reaction mixture was cooled to 0° C. and lithium bis(trimethylsilyl)amine (1 N in THF, 1 mL, 0.957 mmol) was added dropwise. The red solution was stirred at 0° C. for 1 h and then warmed to room temperature. The mixture was quenched with saturated NH4Cl and extracted with EtOAc (3×).The combined organic extracts were washed with satd. NaCl and dried over Na2SO4. The solution was filtered and concentrated in vacuo. The crude product was purified by chromatography through a Redi-Sep pre-packed silica gel column (25 g) eluting with a gradient of 2% to 5% 2 M NH3.MeOH in DCM to give N-(5-methoxypyridin-3-yl)-3-(2-methyl-9-(tetrahydro-2H-pyran-2-yl)-9H-purin-6-yl)pyridin-2-amine (60 mg, 0.144 mmol, 45.0% yield) as an orange solid. m/z (ESI, +ve ion) 418 (M+H)+.

1. customArgon was bubbled for 2 min through a glass microwave reaction vessel
2. customthe tube was sealed
3. temperaturewarmed to room temperature
4. customThe mixture was quenched with saturated NH4Cl
5. extractionextracted with EtOAc (3×).The combined organic extracts
6. washwere washed with satd
7. dry with materialNaCl and dried over Na2SO4
8. filtrationThe solution was filtered
9. concentrationconcentrated in vacuo
10. customThe crude product was purified by chromatography through a Redi-Sep pre-packed silica gel column (25 g)
11. washeluting with a gradient of 2% to 5% 2 M NH3