反应详情

[2′-[(Cyclopropanecarbonyl-ethyl-amino)-methyl]-4′-(6-ethoxy-pyridin-3-yl)-6-methoxy-biphenyl-3-yl]-acetic acid ethyl ester (0.296 g, 0.573 mmol) in tetrahydrofuran (5 mL) and water was treated with lithium hydroxide (0.090 g, 2.14 mmol) and the reaction was stirred at room temperature. When the reaction was complete by analytical LCMS, the mixture was acidified with 1N aqueous hydrochloric acid and extracted three times with ethyl acetate. The combined organic layers were dried and concentrated, and the residue was purified by preparative HPLC to give the title compound.

1. extractionextracted three times with ethyl acetate
2. customThe combined organic layers were dried
3. concentrationconcentrated
4. customthe residue was purified by preparative HPLC