反应详情

Method as example 143 using (S)-1-(2-chloro-4-(3-methylmorpholino)-5H-pyrrolo[3,4-d]pyrimidin-6(7H)-yl)ethanone (intermediate 24) (212 mg, 0.5 mmol) and 1-cyclopropyl-3-(3-fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)urea (intermediate 29) (182 mg, 0.57 mmol) as starting materials. Reaction mixture was partitioned between EtOAc (50 ml) and water (35 ml), the organic layer passed through a hydrophobic frit and concentrated in vacuo to leave a purple solid. The solid was purified by prep. HPLC at high pH to afford a brown/red solid which was re-purified by prep. HPLC at low pH to afford a yellow solid (9 mg, 4%).

1. customReaction mixture
2. customwas partitioned between EtOAc (50 ml) and water (35 ml)
3. concentrationconcentrated in vacuo
4. customto leave a purple solid
5. customThe solid was purified by prep
6. customHPLC at high pH to afford a brown/red solid which
7. customwas re-purified by prep