反应详情

Method as described for example 147 using methyl 2-chloro-4-morpholino-5H-pyrrolo[3,4-d]pyrimidine-6(7H)-carboxylate (intermediate 25) and 1-ethyl-3-(3-fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl) (intermediate 28) as starting materials. The crude reaction mixture was purified by SCX-2 cartridge (loaded in MeOH eluted with 2M methanolic ammonia). Ammonia eluant was concentrated in vacuo. Residue was then purified by prep. HPLC at high pH to afford an off white solid (34.6 mg, 0.08 mmol, 16%).

1. customThe crude reaction mixture
2. customwas purified by SCX-2 cartridge (loaded in MeOH eluted with 2M methanolic ammonia)
3. concentrationAmmonia eluant was concentrated in vacuo
4. customResidue was then purified by prep