Hydrochloric Acid → HCID 67496846, compound

Participants

Reaction Conditions

Reaction Steps

To a 25 ml flask was added ethyl 2-((S)-1-(1-ethyl-3-(pyridin-4-yl)-1H-pyrazol-5-ylamino)-1-oxo-3-phenylpropan-2-ylamino)acetate 101.1.C (105 mg, 0.78 mmole), 0.5 ml of 1N-sodium hydroxide solution and 1.5 ml of methanol. The mixture was stirred at room temperature for 1 hour and at 50° C. for 1 hour. After then, 0.25 ml of 2N-hydrogen chloride solution was added to neutralize. The solvent was removed by rotary evaporation. The crude product was purified by silica gel chromatography to give 101.2 (79 mg, 81% yield) as colorless powder. LCMS ESI (pos.) m/e: 394 (M+H); 1H NMR (400 MHz, DMSO-d6) δ ppm 8.57 (d, J=5.9 Hz, 2H), 7.72 (d, J=5.8 Hz, 2H), 7.34-7.22 (m, 5H), 6.79 (s, 1H), 3.89 (q, J=7.4 Hz, 2H), 3.66 (t, J=7.1 Hz, 1H), 3.30 (d, J=17.2 Hz, 1H), 3.24 (d, J=17.2 Hz, 1H), 3.04-2.93 (m, 2H), 1.24 (t, J=7.2 Hz, 3H).

Provenance & Evidence