HCID 178427446, compound → HCID 178446004, compound

Participants

Reaction Steps

51.0 Milligrams of H-Thr-Asn-Leu-Gln-Glu-Ser-Leu-Arg-Ser-Lys(Tos)-Glu-OH was dissolved in liquid ammonia being dried with metallic sodium previously, then small pieces of metallic sodium were added to the solution under stirring condition until the color of the reaction mixture is changed to blue and kept it for 30 seconds to 1 minute. Further crystals of NH4Cl were added to the reaction mixture, an excess metallic sodium was neutralized, after ammonia was completely removed by distillation at a room temperature, the readtion product was subjected to gel-filtration with Sephadex G-25 gel by using 50%-acetic acid as the eluting solvent, for collecting the desired fraction, and the lyophilizing the raction by adding water after concentration, to obtain 32 mg of the desired product (peptide of the present invention). Hereinafter, this product is referred to as [Peptide F].

Provenance & Evidence