Citric Acid → HCID 131713883, compound

Participants

Reaction Steps

5.32 Grams of Z-Asn-OH was dissolved in 60 ml of tetrahydrofuran, 10 ml of dimethylformamide and 2.04 ml of N-methylmorpholine, to this mixture was added 40 ml of a dimethylformamide solution containing 2.64 ml of isobutyl chloroformate, 3.91 g of H-Leu-OEt.HCl and 2.80 ml of triethylamine and the reaction was carried out by a procedure similar to that described in Reference example 2. After removal of the solvent by distillation, to the residue obtained was added 1M-citric acid, the precipitate formed was collected by filtration, washed with water, dried under a reduced pressure, then was recrystallized from ethyl acetate to obtain 6.1 g of Z-Asn-Leu-OEt.

Provenance & Evidence