反应详情

A mixture of 1.4 g of the product of Example 3 and 5 ml of 50% aqueous formic acid was held on a water bath at 55° C. for 15 minutes and then 5 ml of water were added. The mixture was cooled and vacuum filtered and the filtrate was concentrated after the addition of 5 ml of ethanol. The residue was taken up in 5 ml of ethanol and the mixture was triturated and was vacuum filtered. The product was washed with ethanol and then ether, was vacuum filtered, dried and washed to obtain 0.557 g of pure 7-[{2-amino-4-thiazolyl)-2-methoxyiminoacetyl}amino]-3-[(1-methyl-tetrazol-5-yl)-thiomethyl]-ceph-3-eme-4-carboxylic acid in the syn configuration.

1. temperatureThe mixture was cooled
2. filtrationvacuum filtered
3. concentrationthe filtrate was concentrated
4. additionafter the addition of 5 ml of ethanol
5. customthe mixture was triturated
6. filtrationfiltered
7. washThe product was washed with ethanol
8. filtrationfiltered
9. customdried
10. washwashed