Butyric Acid → Formate

Participants

Reaction Steps

The enzymatic oxidation of glycolic acid in Example 1 was repeated, using 10 mL of an aqueous solution containing glycolic acid (0.25M), aminomethylphosphonic acid (AMPA, 0.20M), FMN (0.01 mM), butyric acid (HPLC internal standard, 0.10M), glycolate oxidase (from spinach, 1.0 IU/mL), and catalase (from Aspergillus niger, 14,000 IU/mL) at pH 8.5. After 6 h, the HPLC yields of glyoxylate, formate, and oxalate were 92.3%, 4.36%, and 5.5%, respectively, and no glycolate remained. The final pH of the reaction mixture was 6.7.

Provenance & Evidence