Butyric Acid → Formate

Participants

Reaction Steps

The enzymatic oxidation of glycolic acid in Example 1 was repeated, using 10 mL of an aqueous solution containing glycolic acid (025M), aminomethylphosphonic acid (AMPA, 0.263M), FMN (0.01 mM), butyric acid (HPLC internal standard, 0.25M), glycolate oxidase (from spinach, 1.0 IU/mL), and catalase (from Aspergillus niger, 14,000 IU/mL) at pH 7.0 and 15° C. After 8 h, the HPLC yields of glyoxylate, formate, and oxalate were 82.8%, 0.9%, and 2.1%, respectively, and 13.9% glycolate remained. The final pH of the reaction mixture was 6.6.

Provenance & Evidence