Sorbitol → aldehydo-D-Xylose

Participants

Reaction Steps

Streptomyces sp. ATCC 21175 was grown under aerobic submerged fermentation conditions at a pH of 7 in a presterilized aqueous medium containing 1 percent sorbitol, 0.75 percent dextrose, sufficient corncob hydrolysate to provide 1 percent xylose, 4 percent steep water at 29° C. and 0.024 percent cobaltous ion. The fermentation was carried out at 30° C., an airflow of 1 volume of air per volume of medium per minute and a back pressure of 10 p.s.i. The fermenting broth was mechanically stirred at 200 r.p.m. and after 65 hours 4 percent filter aid was admixed into the broth and the cellular material harvested from the broth by filtration with suction. The filter cake was washed with demineralized water, broken into small pieces and dried for 5 hours in a forced-air oven at an air temperature of 140° F. The activity of the air-dried filter cake was 660 GIU/g.

Provenance & Evidence