反应详情

5.7 g. 6-Chloro-9-(β-D-ribofuranosyl)-purine, 6 g. N-(2-hydroxyethyl)-2-methoxy-5-chlorobenzylamine and 2.5 g. triethylamine were heated under reflux for 2 hours in 100 ml. n-butanol. Subsequently, the solvent was removed in a vacuum and the residue taken up in 100 ml. chloroform. The chloroform phase was washed several times with water, initially with the addition of some dilute hydrochloric acid and thereafter with a dilute solution of sodium bicarbonate. From the so purified chloroform solution, there crystallized out 4.9 g. (53% of theory) N(6)-(2-hydroxyethyl)-N(6)-(2-methoxy-5-chlorobenzyl)-adenosine, which melted at 98°-100°C.

1. temperatureunder reflux for 2 hours in 100 ml
2. customSubsequently, the solvent was removed in a vacuum
3. washThe chloroform phase was washed several times with water
4. additioninitially with the addition of some dilute hydrochloric acid
5. customFrom the so purified chloroform solution, there crystallized out 4.9 g