Diisopropylcarbodiimide → HCID 59177922, compound

Participants

Reaction Steps

To a round bottom flask was added (S)-8-(4-chlorophenyl)-3-(1-hydroxyethyl)-5-((2-methyl-6-(trifluoromethyl)pyridin-3-yl)methyl)-7-phenyl-[1,2,4]triazolo[4,3-b]pyridazin-6(5H)-one (35 mg, 0.0648 mmol), Boc-L-Valine (15.5 mg, 0.0713 mmol), CH2Cl2(3 ml), DMAP (cat, 3 mg) and DIC (12.3 mg, 0.0973 mmol). The reaction was stirred at rt for 30 min. After this time, the solution was concentrated. The crude product was purified by silica gel column chromatography (8 g silica gel) eluting with a gradient of 0-50% EtOAc/Hex to give product (S)—((S)-1-(8-(4-chlorophenyl)-5-((2-methyl-6-(trifluoromethyl)pyridin-3-yl)methyl)-6-oxo-7-phenyl-5,6-dihydro-[1,2,4]triazolo[4,3-b]pyridazin-3-yl)ethyl) 2-(tert-butoxycarbonylamino)-3-methylbutanoate, as a white solid (39 mg, 81% yield).

Provenance & Evidence