1-Hydroxybenzotriazole → Diisopropylethylamine

Participants

Reaction Steps

The peptide on resin was suspended in DMF (5-7 mL/100 mg resin). In a separate vial, 1.5-2 eq of Glu-DTPE acid was activated by addition of HOBt, and PyBOP in DMF (10% of the volume of peptide mixture) for 5-10 min at pH ≈8 obtained by addition of DIEA (measured with wet pH paper). Activated DTPE acid mixture was added to the solution of peptide and the pH was adjusted to ˜8 with DIEA. The mixture was shaken at RT for 4-18 hours.

Provenance & Evidence