Acetate → HCID 86638819, compound

Participants

Reaction Steps

Batch crystallization was performed by admixing around 300 μL of the protein solution with an equal amount of crystallization solution in a 1.5 mL Eppendorff reaction tube. 300 μL of a particular crystallization solution was prepared by admixing acetate buffer, sodium dihydrogen phosphate stock solution and Milli Q water. In this example, the acetate buffer molarity was 0.1 M, and the acetate buffer pH was around 4.1. Sodium dihydrogen phosphate molarity was around 1.5 M, 1.8 M, 2.1 M and 2.4 M, respectively. The reaction tubes were stored at ambient temperature. Microscopy of 1 μL aliquots was performed after 11 days.

Provenance & Evidence