Acetate → HCID 86638819, compound

Participants

Reaction Steps

Batch crystallization was performed by admixing around 10 mL of protein solution with an equal amount of crystallization solution in a 50 mL Falcon tube. 10 mL of the crystallization solution was prepared by admixing acetate buffer, sodium dihydrogen phosphate stock solution and Milli Q water in the tube. In this example, the acetate buffer molarity was 0.1 M, and the acetate buffer pH was around 4.1. Sodium dihydrogen phosphate molarity was 4.2 M. The tube was stored at ambient temperature, agitating the batch on a laboratory shaker. Microscopy of a 1 μL aliquot of the solution was performed multiple times during the following month.

Provenance & Evidence