Acetate → HCID 86638819, compound

HRID 1663294Notes

Reaction equation

HRID 1663294 reaction equation
[C:1]([O-:4])(=[O:3])[CH3:2].[P:5]([O-:9])([OH:8])([OH:7])=[O:6].[Na+:10]>O>[P:5]([O-:9])([OH:8])([OH:7])=[O:6].[Na+:10].[C:1]([O-:4])(=[O:3])[CH3:2].[Na+:10]

Participants

4

Reactant 2

HCID 175Acetate3×HCID 23672064Monosodium phosphate2×

Product 1

HCID 86638819HCID 86638819, compound

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

Batch crystallization was performed by admixing around 50 mL of protein solution with an equal amount of crystallization solution in a clean 1 L polypropylene bottle. 50 mL of the crystallization solution was prepared by admixing acetate buffer, sodium dihydrogen phosphate stock solution and Milli Q water in the tube. In this example, the acetate buffer molarity was 0.1 M, and the acetate buffer pH was around 4.1. Sodium dihydrogen phosphate molarity was 4.2 M. The container was stored at ambient temperature. Microscopy of a 1 μL aliquot of the solution was performed multiple times during the following month.

Workup

  1. customBatch crystallization
  2. customwith an equal amount of crystallization solution in a clean 1 L polypropylene bottle
  3. customwas stored at ambient temperature

Sources & evidence

ORD record
ord-16c903047be447f09908030f29fa3eb0
Source dataset
uspto-grants-2011_10
Patent number
US08034906B2

Reaction notes

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