Glyoxylic Acid → N,N,N',N'-tetrakis(carboxymethyl)-L-cystine

Participants

Reaction Steps

The alternative route, relying on in-solution phase synthesis of an intermediate product starts with addition of a large excess (40 times) of glyoxylic acid to L-cystine in an alkaline borate buffer. The intermediate product, following pH manipulation and conductivity adjustment of the reaction mixture, purified with ion exchange chromatography to give N,N,N′,N′-tetrakis(carboxymethyl)-L-cystine.

Provenance & Evidence