N,N,N',N'-Tetramethylethylenediamine → HCID 59149716, compound

Participants

Reaction Conditions

Reaction Steps

6-[4-[(1R)-1-(4-Bromophenyl)ethyl]piperazin-1-yl]-3-(trifluoromethyl)[1,2,4]triazolo[4,3-b]pyridazine (obtained as described in Example 306) (250 mg, 0.55 mmol), zinc cyanide (38.7 mg, 0.33 mmol), tris(dibenzylideneacetone)dipalladium(0) (5.0 mg, 5.49 μmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (6.3 mg, 10.98 μmol) and N,N,N′,N′-tetramethylethylenediamine (0.017 mL, 0.11 mmol) were suspended in DMF (2.5 mL) and sealed into a microwave tube. The reaction was heated to 160° C. for 5 minutes in a microwave reactor and cooled to ambient temperature. The resulting mixture was applied to an SCX column and eluted with methanol followed by 2M ammonia in methanol. Fractions containing product were combined and evaporated then purified by preparative HPLC (Waters XTerra C18 column, 5μ silica, 19 mm diameter, 100 mm length), using decreasingly polar mixtures of water (containing 1% ammonia) and MeCN as eluents. Fractions containing the desired compound were evaporated to dryness to afford 4-[(1R)-1-[4-[3-(trifluoromethyl)[1,2,4]triazolo[4,3-b]pyridazin-6-yl]piperazin-1-yl]ethyl]benzonitrile (168 mg, 76%) as a colourless solid.

Provenance & Evidence